Composite

Part:BBa_K3015001

Designed by: Valentina Stuchlik   Group: iGEM19_BOKU-Vienna   (2019-10-13)


pMyco-RBS

This is the Mycolactone-Riboswitch BBa_K3015000 from BOKU-Vienna fused to the Promotor BBa_J23105 on the 5' and to the RBS BBa_B0034 on the 3'. This part can be placed upstream of a gene of interest in order to up-regulate the expression of downstream genes after induction with the target - Mycolactone (e.g. BBa_K3015002)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None